Fluorescent nucleobase analogues for base–base FRET in nucleic acids: synthesis, photophysics and applications

• Mattias Bood,
• Sangamesh Sarangamath,
• Moa S. Wranne,
• Morten Grøtli and
• L. Marcus Wilhelmsson

Beilstein J. Org. Chem. 2018, 14, 114–129, doi:10.3762/bjoc.14.7

• distance window of opportunity than external fluorophores, and, thus, have the potential to facilitate better structure resolution. Netropsin DNA binding and the B-to-Z-DNA transition are examples of structure investigations that recently have been performed using base–base FRET and that are described here

• . Keywords: B-to-Z-DNA transition; fluorescent base analogues; FRET; netropsin; nucleic acid structure and dynamics; quadracyclic adenines; tricyclic cytosines; Z-DNA; Review Introduction The importance of nucleic acid structure and dynamics in the understanding of vital processes in living organisms has

• , respectively). The results show significant changes in the FRET efficiencies upon B-to-Z-DNA transition (e.g., from 35 to 8%) that can, not only, be used to monitor the presence of Z-form DNA but also to determine the rate constants for these transitions [73]. We showed in this investigation that the FRET